• Immunohistochemistry analysis of paraffin-embedded human lung cancer (A) , thyroid cancer (B) , lymph node (C) and brain (D) showing cytoplasmic and extracellular matrix localization with DAB staining using Wnt-5a monoclonal antibody.
  • Confocal immunofluorescence analysis of PC-12 cells using Wnt-5a monoclonal antibody (green) , showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
  • Western blot analysis using Wnt-5a monoclonal antibody against HEK293 (1) and Wnt-5a-hIgGFc transfected HEK293 cell lysate (2).

Anti-WNT5A antibody [6F2] (STJ98453)

SKU:
STJ98453

Current Stock:
Host: Mouse
Applications: WB/IHC/IF/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Protein Wnt-5a is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Monoclonal
Clone ID: 6F2
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WNT5A
Gene ID: 7474
Uniprot ID: WNT5A_HUMAN
Specificity: Wnt-5a Monoclonal Antibody detects endogenous levels of Wnt-5a protein.
Immunogen: Purified recombinant fragment of Wnt-5a expressed in E. Coli.
Post Translational Modifications Glycosylation is necessary for secretion but not for activity. Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition. Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT5A.
Function Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
Protein Name Protein Wnt-5a
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-3238698
Reactome: R-HSA-373080
Reactome: R-HSA-3772470
Reactome: R-HSA-4086398
Reactome: R-HSA-4086400
Reactome: R-HSA-4608870
Reactome: R-HSA-5099900
Reactome: R-HSA-5140745
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-9673324
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Protein Wnt-5a antibody
Anti-WNT5A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance