• STJ70144 (1µg/ml) staining of Jurkat (A) and negative control U937 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70144 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ70144 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Anti-WNT4 antibody (Internal) (STJ70144)

SKU:
STJ70144-100

£303.50
Shipping:
Free Shipping
Current Stock:
Host: Goat
Applications: Pep-ELISA/WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-WNT4 (Internal) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
IF-Strong expression of the protein seen in the vesicles of HeLa and MCF7 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:4000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: WNT4
Gene ID: 54361
Uniprot ID: WNT4_HUMAN
Immunogen Region: Internal
Accession Number: NP_110388.2
Immunogen Sequence: SNWLYLAKLSSVGS
Function Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Plays an important role in the embryonic development of the urogenital tract and the lung. Required for normal mesenchyme to epithelium transition during embryonic kidney development. Required for the formation of early epithelial renal vesicles during kidney development. Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries. Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary. Required for normal lung development and for normal patterning of trachael cartilage rings.
Protein Name Protein Wnt-4
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-3238698
Reactome: R-HSA-373080
Reactome: R-HSA-3772470
Reactome: R-HSA-4086400
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Protein Wnt-4 antibody
Anti-WNT4 antibody
Anti-UNQ426 antibody
Anti-PRO864 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance