• Immunohistochemistry analysis of paraffin-embedded human normal stomach (A) , normal liver (B) , normal kidney (C) and rectum cancer tissues (D) with DAB staining using Wnt-10b monoclonal antibody.
  • Confocal immunofluorescence analysis of PANC-1 cells using Wnt-10b monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • Western blot analysis using Wnt-10b monoclonal antibody against HeLa cell lysate (1).

Anti-WNT10B antibody [5A7] (STJ98452)

SKU:
STJ98452

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Protein Wnt-10b is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Monoclonal
Clone ID: 5A7
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WNT10B
Gene ID: 7480
Uniprot ID: WN10B_HUMAN
Specificity: Wnt-10b Monoclonal Antibody detects endogenous levels of Wnt-10b protein.
Immunogen: Purified recombinant fragment of human Wnt-10b expressed in E. Coli.
Post Translational Modifications Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
Function Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin.
Protein Name Protein Wnt-10b
Protein Wnt-12
Database Links Reactome: R-HSA-3238698
Reactome: R-HSA-373080
Reactome: R-HSA-381340
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Protein Wnt-10b antibody
Anti-Protein Wnt-12 antibody
Anti-WNT10B antibody
Anti-WNT12 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance