• Immunofluorescence analysis of PC-12 cells using WNT1 Rabbit polyclonal antibody (STJ111158) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using WNT1 Rabbit polyclonal antibody (STJ111158) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using WNT1 Rabbit polyclonal antibody (STJ111158) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using WNT1 Rabbit polyclonal antibody (STJ111158) at 1:8000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 60s.

Anti-WNT1 antibody (231-370) (STJ111158)

SKU:
STJ111158

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WNT1 (231-370) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:4000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WNT1
Gene ID: 7471
Uniprot ID: WNT1_HUMAN
Immunogen Region: 231-370
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 231-370 of human WNT1 (NP_005421.1).
Immunogen Sequence: TCWMRLPTLRAVGDVLRDRF DGASRVLYGNRGSNRASRAE LLRLEPEDPAHKPPSPHDLV YFEKSPNFCTYSGRLGTAGT AGRACNSSSPALDGCELLCC GRGHRTRTQRVTERCNCTFH WCCHVSCRNCTHTRVLHECL
Post Translational Modifications Palmitoleoylation is required for efficient binding to frizzled receptors. Palmitoleoylation is necessary for proper trafficking to cell surface (Probable). Depalmitoleoylated by NOTUM, leading to inhibit Wnt signaling pathway.
Function Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Plays an essential role in the development of the embryonic brain and central nervous system (CNS). Has a role in osteoblast function, bone development and bone homeostasis.
Protein Name Proto-Oncogene Wnt-1
Proto-Oncogene Int-1 Homolog
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-3238698
Reactome: R-HSA-373080
Reactome: R-HSA-381340
Reactome: R-HSA-4086400
Reactome: R-HSA-4641262
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Proto-Oncogene Wnt-1 antibody
Anti-Proto-Oncogene Int-1 Homolog antibody
Anti-WNT1 antibody
Anti-INT1 antibody

Information sourced from Uniprot.org

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