• Western blot analysis of extracts of Mouse kidney, using WNK1 Rabbit monoclonal antibody (STJ11102021) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-WNK1 antibody (1-100) [S1MR] (STJ11102021)

SKU:
STJ11102021

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-WNK1 (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WNK1
Gene ID: 65125
Uniprot ID: WNK1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human WNK1 (Q9H4A3).
Immunogen Sequence: MSGGAAEKQSSTPGSLFLSP PAPAPKNGSSSDSSVGEKLG AAAADAVTGRTEEYRRRRHT MDKDSRGAAATTTTTEHRFF RRSVICDSNATALELPGLPL
Tissue Specificity Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 1: Strong expression in dorsal root ganglia and spinal cord. Isoform 3: This isoform is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
Post Translational Modifications Autophosphorylated at Ser-378 and Ser-382, promoting its activity. Autophosphorylation at Ser-382 is inhibited by intracellular calcium. Phosphorylation at Thr-60 increases ability to activate SGK1. Ubiquitinated by the BCR(KLHL3) complex, leading to its degradation. Also ubiquitinated by the BCR(KLHL2) complex. May be O-glycosylated.
Function Serine/threonine-protein kinase component of the WNK1-SPAK/OSR1 kinase cascade, which acts as a key regulator of blood pressure and regulatory volume increase by promoting ion influx. WNK1 mediates regulatory volume increase in response to hyperosmotic stress by acting as a molecular crowding sensor, which senses cell shrinkage and mediates formation of a membraneless compartment by undergoing liquid-liquid phase separation. The membraneless compartment concentrates WNK1 with its substrates, OXSR1/OSR1 and STK39/SPAK, promoting WNK1-dependent phosphorylation and activation of downstream kinases OXSR1/OSR1 and STK39/SPAK. Following activation, OXSR1/OSR1 and STK39/SPAK catalyze phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A5/KCC2 and SLC12A6/KCC3, regulating their activity. Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx.simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux. Also acts as a regulator of angiogenesis in endothelial cells via activation of OXSR1/OSR1 and STK39/SPAK: activation of OXSR1/OSR1 regulates chemotaxis and invasion, while STK39/SPAK regulates endothelial cell proliferation. Also acts independently of the WNK1-SPAK/OSR1 kinase cascade by catalyzing phosphorylation of other substrates, such as SYT2, PCF11 and NEDD4L. Mediates phosphorylation of SYT2, regulating SYT2 association with phospholipids and membrane-binding. Regulates mRNA export in the nucleus by mediating phosphorylation of PCF11, thereby decreasing the association between PCF11 and POLR2A/RNA polymerase II and promoting mRNA export to the cytoplasm. Acts as a negative regulator of autophagy. Required for the abscission step during mitosis, independently of the WNK1-SPAK/OSR1 kinase cascade. May also play a role in actin cytoskeletal reorganization. Also acts as a scaffold protein independently of its protein kinase activity: negatively regulates cell membrane localization of various transporters and channels, such as SLC4A4, SLC26A6, SLC26A9, TRPV4 and CFTR. Involved in the regulation of epithelial Na(+) channel (ENaC) by promoting activation of SGK1 in a kinase-independent manner: probably acts as a scaffold protein that promotes the recruitment of SGK1 to the mTORC2 complex in response to chloride, leading to mTORC2-dependent phosphorylation and activation of SGK1. Acts as an assembly factor for the ER membrane protein complex independently of its protein kinase activity: associates with EMC2 in the cytoplasm via its amphipathic alpha-helix, and prevents EMC2 ubiquitination and subsequent degradation, thereby promoting EMC2 stabilization. Isoform 3: Kinase-defective isoform specifically expressed in kidney, which acts as a dominant-negative regulator of the longer isoform 1. Does not directly inhibit WNK4 and has no direct effect on sodium and chloride ion transport. Down-regulates sodium-chloride cotransporter activity indirectly by inhibiting isoform 1, it associates with isoform 1 and attenuates its kinase activity. In kidney, may play an important role regulating sodium and potassium balance.
Protein Name Serine/Threonine-Protein Kinase Wnk1
Erythrocyte 65 Kda Protein
P65
Kinase Deficient Protein
Protein Kinase Lysine-Deficient 1
Protein Kinase With No Lysine 1
Hwnk1
Database Links Reactome: R-HSA-2672351
Cellular Localisation Cytoplasm
Nucleus
Cytoskeleton
Spindle
Mediates Formation And Localizes To Cytoplasmic Membraneless Compartment In Response To Hyperosmotic Stress
Also Localizes To The Nucleus
Localizes To The Mitotic Spindle During Mitosis
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Wnk1 antibody
Anti-Erythrocyte 65 Kda Protein antibody
Anti-P65 antibody
Anti-Kinase Deficient Protein antibody
Anti-Protein Kinase Lysine-Deficient 1 antibody
Anti-Protein Kinase With No Lysine 1 antibody
Anti-Hwnk1 antibody
Anti-WNK1 antibody
Anti-HSN2 antibody
Anti-KDP antibody
Anti-KIAA0344 antibody
Anti-PRKWNK1 antibody

Information sourced from Uniprot.org

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