| Host: | Goat |
| Applications: | Pep-ELISA/WB/IF/FC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-WISP1 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 32-35kDa band observed in lysates of cell line CaCo-2 and approx. 40kDa in Human Tonsil lysates, and additionally in a lysate of cell line CaCo-2, which were successfully block |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | CCN4 |
| Gene ID: | 8840 |
| Uniprot ID: | CCN4_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_003873.1; NP_543028.1 |
| Specificity: | This antibody is expected to recognize both reported isoforms (NP_003873.1; NP_543028.1). |
| Immunogen Sequence: | ESYPDFSEIAN |
| Function | Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. |
| Protein Name | Ccn Family Member 4Wnt1-Inducible-Signaling Pathway Protein 1Wisp-1Wnt-1-Induced Secreted Protein |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Ccn Family Member 4 antibodyAnti-Wnt1-Inducible-Signaling Pathway Protein 1 antibodyAnti-Wisp-1 antibodyAnti-Wnt-1-Induced Secreted Protein antibodyAnti-CCN4 antibodyAnti-WISP1 antibody |
Information sourced from Uniprot.org