• STJ71370 (0. 1µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71370 (0. 3µg/ml) staining of Caco-2 cell lysate 1 (A) and Caco-2 cell lysate 2 (B) + petide (C). (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71370 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71370 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71370 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-WISP1 antibody (C-Term) (STJ71370)

SKU:
STJ71370-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-WISP1 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CCN4
Gene ID: 8840
Uniprot ID: CCN4_HUMAN
Immunogen Region: C-Term
Accession Number: NP_003873.1; NP_543028.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_003873.1; NP_543028.1).
Immunogen Sequence: ESYPDFSEIAN
Function Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan.
Protein Name Ccn Family Member 4
Wnt1-Inducible-Signaling Pathway Protein 1
Wisp-1
Wnt-1-Induced Secreted Protein
Cellular Localisation Secreted
Alternative Antibody Names Anti-Ccn Family Member 4 antibody
Anti-Wnt1-Inducible-Signaling Pathway Protein 1 antibody
Anti-Wisp-1 antibody
Anti-Wnt-1-Induced Secreted Protein antibody
Anti-CCN4 antibody
Anti-WISP1 antibody

Information sourced from Uniprot.org

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