Anti-WIPI2 antibody (187-436) (STJ29675)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal WIPI2 (187-436) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | WIPI2 |
| Gene ID | 26100 |
| Uniprot ID | WIPI2_HUMAN |
| Immunogen Region | 187-436 |
| Immunogen Sequence | LAALAFDASGTKLATASEKG TVIRVFSIPEGQKLFEFRRG VKRCVSICSLAFSMDGMFLS ASSNTETVHIFKLETVKEKP PEEPTTWTGYFGKVLMASTS YLPSQVTEMFNQGRAFATVR LPFCGHKNICSLATIQKIPR LLVGAADGYLYMYNLDPQEG GECALMKQHRLDGSLETTNE ILDSASHDCPLVTQTYGAAA GKGTYVPSSPTRLAYTDDLG AVGGACLEDEASALRLDED |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 187-436 of human WIPI2 (NP_057087.2). |
Additional Info
| Tissue Specificity | Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors. |
| Function | Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Involved in an early step of the formation of preautophagosomal structures. Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases. Mediates ER-isolation membranes contacts by interacting with the ULK1:RB1CC1 complex and PtdIns3P. Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane. Isoform 4: Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes and preautophagosomal structures, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Isoform 4 is also required for autophagic clearance of pathogenic bacteria. Isoform 4 binds the membrane surrounding Salmonella and recruits the ATG12-5-16L1 complex, initiating LC3 conjugation, autophagosomal membrane formation, and engulfment of Salmonella. |
| Protein Name | Wd Repeat Domain Phosphoinositide-Interacting Protein 2Wipi-2Wipi49-Like Protein 2 |
| Database Links | Reactome: R-HSA-1632852 |
| Cellular Localisation | Preautophagosomal Structure MembranePeripheral Membrane ProteinCytoplasmic SideLocalizes To Omegasomes Membranes Which Are Endoplasmic Reticulum Connected Structures At The Origin Of Preautophagosomal StructuresEnriched At Preautophagosomal Structure Membranes In Response To Ptdins3p |
| Alternative Antibody Names | Anti-Wd Repeat Domain Phosphoinositide-Interacting Protein 2 antibodyAnti-Wipi-2 antibodyAnti-Wipi49-Like Protein 2 antibodyAnti-WIPI2 antibodyAnti-CGI-50 antibody |
Information sourced from Uniprot.org