| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-WIPI2 (187-436) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
WIPI2 |
| Gene ID: |
26100 |
| Uniprot ID: |
WIPI2_HUMAN |
| Immunogen Region: |
187-436 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 187-436 of human WIPI2 (NP_057087.2). |
| Immunogen Sequence: |
LAALAFDASGTKLATASEKG TVIRVFSIPEGQKLFEFRRG VKRCVSICSLAFSMDGMFLS ASSNTETVHIFKLETVKEKP PEEPTTWTGYFGKVLMASTS YLPSQVTEMFNQGRAFATVR LPFCGHKNICSLATIQKIPR LLVGAADGYLYMYNLDPQEG GECALMKQHRLDGSLETTNE ILDSASHDCPLVTQTYGAAA GKGTYVPSSPTRLAYTDDLG AVGGACLEDEASALRLDED |
| Tissue Specificity | Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors. |
| Function | Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Involved in an early step of the formation of preautophagosomal structures. Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases. Mediates ER-isolation membranes contacts by interacting with the ULK1:RB1CC1 complex and PtdIns3P. Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane. Isoform 4: Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes and preautophagosomal structures, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Isoform 4 is also required for autophagic clearance of pathogenic bacteria. Isoform 4 binds the membrane surrounding Salmonella and recruits the ATG12-5-16L1 complex, initiating LC3 conjugation, autophagosomal membrane formation, and engulfment of Salmonella. |
| Protein Name | Wd Repeat Domain Phosphoinositide-Interacting Protein 2Wipi-2Wipi49-Like Protein 2 |
| Database Links | Reactome: R-HSA-1632852 |
| Cellular Localisation | Preautophagosomal Structure MembranePeripheral Membrane ProteinCytoplasmic SideLocalizes To Omegasomes Membranes Which Are Endoplasmic Reticulum Connected Structures At The Origin Of Preautophagosomal StructuresEnriched At Preautophagosomal Structure Membranes In Response To Ptdins3p |
| Alternative Antibody Names | Anti-Wd Repeat Domain Phosphoinositide-Interacting Protein 2 antibodyAnti-Wipi-2 antibodyAnti-Wipi49-Like Protein 2 antibodyAnti-WIPI2 antibodyAnti-CGI-50 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
