• Immunofluorescence analysis of PC-12 cells using WIF1 Rabbit polyclonal antibody (STJ27339) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using WIF1 Rabbit polyclonal antibody (STJ27339) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of HeLa, using WIF1 antibody (STJ27339) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-WIF1 antibody (307-377) (STJ27339)

SKU:
STJ27339

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WIF1 (307-377) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WIF1
Gene ID: 11197
Uniprot ID: WIF1_HUMAN
Immunogen Region: 307-377
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 307-377 of human WIF1 (NP_009122.2).
Immunogen Sequence: KPVCEPGCGAHGTCHEPNKC QCQEGWHGRHCNKRYEASLI HALRPAGAQLRQHTPSLKKA EERRDPPESNY
Function Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
Protein Name Wnt Inhibitory Factor 1
Wif-1
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-3772470
Cellular Localisation Secreted
Alternative Antibody Names Anti-Wnt Inhibitory Factor 1 antibody
Anti-Wif-1 antibody
Anti-WIF1 antibody
Anti-UNQ191 antibody
Anti-PRO217 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance