• Immunohistochemistry analysis of WIF1 in paraffin-embedded Human breast cancer using WIF1 Rabbit polyclonal antibody (STJ114835) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and 293T cells transfected with WIF1 using WIF1 Rabbit polyclonal antibody (STJ114835) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-WIF1 antibody (29-220) (STJ114835)

SKU:
STJ114835

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WIF1 (29-220) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WIF1
Gene ID: 11197
Uniprot ID: WIF1_HUMAN
Immunogen Region: 29-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-220 of human WIF1 (NP_009122.2).
Immunogen Sequence: GPPQEESLYLWIDAHQARVL IGFEEDILIVSEGKMAPFTH DFRKAQQRMPAIPVNIHSMN FTWQAAGQAEYFYEFLSLRS LDKGIMADPTVNVPLLGTVP HKASVVQVGFPCLGKQDGVA AFEVDVIVMNSEGNTILQTP QNAIFFKTCQQAECPGGCRN GGFCNERRICECPDGFHGPH CEKALCTPRCMN
Function Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
Protein Name Wnt Inhibitory Factor 1
Wif-1
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-3772470
Cellular Localisation Secreted
Alternative Antibody Names Anti-Wnt Inhibitory Factor 1 antibody
Anti-Wif-1 antibody
Anti-WIF1 antibody
Anti-UNQ191 antibody
Anti-PRO217 antibody

Information sourced from Uniprot.org

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