| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | WFS1 |
| Gene ID: | 7466 |
| Uniprot ID: | WFS1_HUMAN |
| Immunogen Region: | 1-285 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human WFS1 (NP_005996.2). |
| Immunogen Sequence: | MDSNTAPLGPSCPQPPPAPQ PQARSRLNATASLEQERSER PRAPGPQAGPGPGVRDAAAP AEPQAQHTRSRERADGTGPT KGDMEIPFEEVLERAKAGDP KAQTEVGKHYLQLAGDTDEE LNSCTAVDWLVLAAKQGRRE AVKLLRRCLADRRGITSENE REVRQLSSETDLERAVRKAA LVMYWKLNPKKKKQVAVAEL LENVGQVNEHDGGAQPGPVP KSLQKQRRMLERLVSSESK |
| Tissue Specificity | Highly expressed in heart followed by brain, placenta, lung and pancreas. Weakly expressed in liver, kidney and skeletal muscle. Also expressed in islet and beta-cell insulinoma cell line. |
| Function | Participates in the regulation of cellular Ca(2+) homeostasis, at least partly, by modulating the filling state of the endoplasmic reticulum Ca(2+) store. Negatively regulates the ER stress response and positively regulates the stability of V-ATPase subunits ATP6V1A and ATP1B1 by preventing their degradation through an unknown proteasome-independent mechanism. |
| Protein Name | Wolframin |
| Database Links | Reactome: R-HSA-381038Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinCytoplasmic VesicleSecretory VesicleCo-Localizes With Atp6v1a In The Secretory Granules In Neuroblastoma Cell Lines |
| Alternative Antibody Names | Anti-Wolframin antibodyAnti-WFS1 antibody |
Information sourced from Uniprot.org