• Western blot analysis of lysates from wild type (WT) and 293T cells transfected with TET3 using TET3 Rabbit polyclonal antibody (STJ11103351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-TET3 antibody (1-285) (STJ11103351)

SKU:
STJ11103351

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TET3 (1-285) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Tet3
Gene ID: 194388
Uniprot ID: TET3_MOUSE
Immunogen Region: 1-285
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of mouse TET3 (Q8BG87).
Immunogen Sequence: MSQFQVPLAVQPDLSGLYDF PQGQVMVGGFQGPGLPMAGS ETQLRGGGDGRKKRKRCGTC DPCRRLENCGSCTSCTNRRT HQICKLRKCEVLKKKAGLLK EVEINAREGTGPWAQGATVK TGSELSPVDGPVPGQMDSGP VYHGDSRQLSTSGAPVNGAR EPAGPGLLGAAGPWRVDQKP DWEAASGPTHAARLEDAHDL VAFSAVAEAVSSYGALSTRL YETFNREMSREAGSNGRGP
Tissue Specificity Highly expressed in germinal vesicle (GV) stage and MII-stage oocytes and in early embryos.
Post Translational Modifications Monoubiquitinated at Lys-1002 by the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex called CRL4(VprBP) or CUL4A-RBX1-DDB1-DCAF1/VPRBP complex.this modification promotes binding to DNA.
Function Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG.
Protein Name Methylcytosine Dioxygenase Tet3
Cellular Localisation Nucleus
Chromosome
Cytoplasm
At The Zygotic Stage
Localizes In The Male And Female Pronucleus
While It Localizes To The Cytoplasm At Other Preimplantation Stages
Binds To The Promoter Of Target Genes
Close To The Transcription Start Site
Alternative Antibody Names Anti-Methylcytosine Dioxygenase Tet3 antibody
Anti-Tet3 antibody

Information sourced from Uniprot.org

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