Anti-WEE1 antibody (1-100) [S4785RM] (STJ11104785)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Wee1-Like Protein Kinase (1-100) antibody for use in IHC-P and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S4785RM |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | WEE1 |
| Gene ID | 7465 |
| Uniprot ID | WEE1_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MSFLSRQQPPPPRRAGAACT LRQKLIFSPCSDCEEEEEEE EEEGSGHSTGEDSAFQEPDS PLPPARSPTEPGPERRRSPG PAPGSPGELEEDLLLPGACP |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human WEE1 (NP_003381.1). |
Additional Info
| Post Translational Modifications | Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase. Dephosphorylated at Thr-239 by CTDP1. Dephosphorylated at Ser-53 and Ser-123 by the serine/threonine-protein phosphatase 2A preventing its ubiquitin-mediated degradation. Ubiquitinated and degraded at the onset of G2/M phase. |
| Function | Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. |
| Protein Name | Wee1-Like Protein KinaseWee1huWee1a Kinase |
| Database Links | Reactome: R-HSA-156711Reactome: R-HSA-69202Reactome: R-HSA-69273Reactome: R-HSA-69478Reactome: R-HSA-69656Reactome: R-HSA-75035Reactome: R-HSA-983231 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Wee1-Like Protein Kinase antibodyAnti-Wee1hu antibodyAnti-Wee1a Kinase antibodyAnti-WEE1 antibody |
Information sourced from Uniprot.org