Anti-WDR44 antibody (STJ191635)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from part of the human protein
General Information
| Short Description | Rabbit polyclonal anti-WD repeat-containing protein 44 for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | WDR44 |
| Gene ID | 54521 |
| Uniprot ID | WDR44_HUMAN |
| Immunogen | Synthesized peptide derived from part of the human protein |
| Specificity | WDR44 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Function | Downstream effector for Rab11 which regulates Rab11 intracellular membrane trafficking functions such as endocytic recycling, intracellular ciliogenesis and protein export. ATK1-mediated phosphorylation of WDR44 induces binding to Rab11 which activates endocytic recycling of transferrin receptor back to the plasma membrane. When bound to Rab11, prevents the formation of the ciliogenic Rab11-Rabin8/RAB3IP-RAB11FIP3 complex, therefore inhibiting preciliary trafficking and ciliogenesis. Participates in neo-synthesized protein export by connecting the endoplasmic reticulum (ER) with the endosomal tubule via direct interactions with the integral ER proteins VAPA or VAPB and the endosomal protein GRAFs (GRAF1/ARHGAP26 or GRAF2/ARHGAP10), which facilitates the transfer of proteins such as E-cadherin, MPP14 and CFTR into a Rab8-Rab10-Rab11-dependent export route. |
| Protein Name | Wd Repeat-Containing Protein 44Rab11-Binding ProteinRab11bpRabphilin-11 |
| Database Links | |
| Cellular Localisation | CytoplasmCytosolPerinuclear RegionEndosome MembraneGolgi ApparatusTrans-Golgi NetworkColocalized With Rab11aRab8aRab10 And Mical1 On Endosomal TubulesColocalized With Rab11a Along Microtubules Oriented Toward Lamellipodia |
| Alternative Antibody Names | Anti-Wd Repeat-Containing Protein 44 antibodyAnti-Rab11-Binding Protein antibodyAnti-Rab11bp antibodyAnti-Rabphilin-11 antibodyAnti-WDR44 antibodyAnti-RPH11 antibody |
Information sourced from Uniprot.org