Anti-WASH antibody (C-Term) (STJA0041457)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenWASH synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human WASH. This sequence is highly conserved in rat, mouse, and chicken WASH, and has low homology to other WASP family members.
General Information
| Short Description | Rabbit polyclonal anti-WASH (C-Term) for use in ELISA, WB and ICC in Chicken, Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC |
| Host | Rabbit |
| Reactivity | Chicken/Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000ICC 1:200 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20ยฐC is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20ยฐC. |
Target Information
| Gene Symbol | WASHC1 |
| Gene ID | 100287171 |
| Uniprot ID | WASH1_HUMAN |
| Immunogen | WASH synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human WASH. This sequence is highly conserved in rat, mouse, and chicken WASH, and has low homology to other WASP family members. |
| Immunogen Region | C-Term |
| Immunogen Sequence | Human |
| Specificity | The antibody detects a 70 kDa* protein corresponding to the molecular mass of WASH on SDS-PAGE immunoblots of human Jurkat cells. |
Additional Info
| Post Translational Modifications | Ubiquitinated at Lys-220 via 'Lys-63'-linked ubiquitin chains by the TRIM27:MAGEL2 E3 ubiquitin ligase complex, leading to promote endosomal F-actin assembly. |
| Function | Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Involved in endocytic trafficking of EGF. Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration. In T-cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL.proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation. Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex. Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity. |
| Protein Name | Wash Complex Subunit 1Cxyorf1-Like Protein On Chromosome 9Protein Fam39eWas Protein Family Homolog 1 |
| Database Links | |
| Cellular Localisation | Early Endosome MembraneRecycling Endosome MembraneLate EndosomeCytoplasmic VesicleAutophagosomeCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleLocalization To The Endosome Membrane Is Mediated Via Its Interaction With Washc2Localizes To Mmp14-Positive Late Endosomes And Transiently To InvadipodiaLocalized To Salmonella Typhimurium Entry Sites |
| Alternative Antibody Names | Anti-Wash Complex Subunit 1 antibodyAnti-Cxyorf1-Like Protein On Chromosome 9 antibodyAnti-Protein Fam39e antibodyAnti-Was Protein Family Homolog 1 antibodyAnti-WASHC1 antibodyAnti-FAM39E antibodyAnti-WASH1 antibody |
Information sourced from Uniprot.org