Anti-WASF1/WAVE antibody (Internal) (STJ72104)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ72104-100
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General Information

Short DescriptionGoat polyclonal antibody anti-WASF1/WAVE (Internal) is suitable for use in ELISA research applications.
ApplicationsPep-ELISA
HostGoat
ReactivityHuman/Mouse/Rat/Dog/Cow
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:2000.
WB: Preliminary experiments in Human Brain lysates of different regions gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolWASF1
Gene ID8936
Uniprot IDWASF1_HUMAN
Accession NumberNP_003922.1
Immunogen RegionInternal
Immunogen SequenceRKQKQKNLDRPHEP
SpecificityReported variants represent identical protein: NP_001020106.1, NP_003922.1, NP_001020107.1, NP_001020105.1

Additional Info

Post Translational Modifications Phosphorylated on tyrosine residues by ABL1 and dephosphorylated by PTPN12.
Function Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes. Also involved in the regulation of mitochondrial dynamics.
Protein Name Actin-Binding Protein Wasf1
Protein Wave-1
Verprolin Homology Domain-Containing Protein 1
Wiskott-Aldrich Syndrome Protein Family Member 1
Wasp Family Protein Member 1
Database Links Reactome: R-HSA-2029482
Reactome: R-HSA-4420097
Reactome: R-HSA-5663213
Reactome: R-HSA-9013149
Reactome: R-HSA-9013423
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Cytoskeleton
Synapse
Cell Junction
Focal Adhesion
Dot-Like Pattern In The Cytoplasm
Concentrated In Rac-Regulated Membrane-Ruffling Areas
Partial Translocation To Focal Adhesion Sites Might Be Mediated By Interaction With Sorbs2
In Neurons
Colocalizes With Activated Ntrk2 After Bdnf Addition In Endocytic Sites Through The Association With Tmem108
Alternative Antibody Names Anti-Actin-Binding Protein Wasf1 antibody
Anti-Protein Wave-1 antibody
Anti-Verprolin Homology Domain-Containing Protein 1 antibody
Anti-Wiskott-Aldrich Syndrome Protein Family Member 1 antibody
Anti-Wasp Family Protein Member 1 antibody
Anti-WASF1 antibody
Anti-KIAA0269 antibody
Anti-SCAR1 antibody
Anti-WAVE1 antibody

Information sourced from Uniprot.org

Citations

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