• Immunofluorescence analysis of NIH-3T3 cells using WAPL Rabbit monoclonal antibody (STJ11103590) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using WAPL Rabbit monoclonal antibody (STJ11103590) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of C2C12 cells, using WAPL Rabbit monoclonal antibody (STJ11103590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using WAPL Rabbit monoclonal antibody (STJ11103590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-WAPL antibody (1-100) [S0MR] (STJ11103590)

SKU:
STJ11103590

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-WAPL (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WAPL
Gene ID: 23063
Uniprot ID: WAPL_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human WAPL (Q7Z5K2).
Immunogen Sequence: MTSRFGKTYSRKGGNGSSKF DEVFSNKRTTLSTKWGETTF MAKLGQKRPNFKPDIQEIPK KPKVEEESTGDPFGFDSDDE SLPVSSKNLAQVKCSSYSES
Tissue Specificity Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart.
Post Translational Modifications Deubiquitinated by USP37.leading to stabilization.
Function Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
Protein Name Wings Apart-Like Protein Homolog
Friend Of Ebna2 Protein
Wapl Cohesin Release Factor
Database Links Reactome: R-HSA-2467813
Reactome: R-HSA-2468052
Reactome: R-HSA-2470946
Reactome: R-HSA-2500257
Cellular Localisation Isoform 2: Nucleus
Nucleus
Chromosome
Cytoplasm
Associates With Chromatin Through The Cohesin Complex During Interphase
Released In The Cytoplasm From Nuclear Envelope Breakdown Until Anaphase
It Reaccumulates In Nucleus At Telophase
Alternative Antibody Names Anti-Wings Apart-Like Protein Homolog antibody
Anti-Friend Of Ebna2 Protein antibody
Anti-Wapl Cohesin Release Factor antibody
Anti-WAPL antibody
Anti-FOE antibody
Anti-KIAA0261 antibody
Anti-WAPAL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance