• Immunohistochemistry analysis of paraffin-embedded mouse leydig cells using Vitronectin antibody (STJ26103) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using Vitronectin antibody (STJ26103) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Vitronectin antibody (STJ26103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-VTN antibody (364-478) (STJ26103)

SKU:
STJ26103

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VTN (364-478) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VTN
Gene ID: 7448
Uniprot ID: VTNC_HUMAN
Immunogen Region: 364-478
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 364-478 of human Vitronectin (NP_000629.3).
Immunogen Sequence: SLAKKQRFRHRNRKGYRSQR GHSRGRNQNSRRPSRATWLS LFSSEESNLGANNYDDYRMD WLVPATCEPIQSVFFFSGDK YYRVNLRTRRVDTVDPPYPR SIAQYWLGCPAPGHL
Tissue Specificity Expressed in the retina pigment epithelium (at protein level). Expressed in plasma (at protein level). Expressed in serum (at protein level).
Post Translational Modifications Sulfated on tyrosine residues. N- and O-glycosylated. Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading. It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both. Phosphorylation sites are present in the extracellular medium.
Function Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
Protein Name Vitronectin
Vn
S-Protein
Serum-Spreading Factor
V75 Cleaved Into - Vitronectin V65 Subunit - Vitronectin V10 Subunit - Somatomedin-B
Database Links Reactome: R-HSA-2129379
Reactome: R-HSA-216083
Reactome: R-HSA-3000170
Reactome: R-HSA-3000178
Reactome: R-HSA-977606
Cellular Localisation Secreted
Extracellular Space
Parasitophorous Vacuole
(Microbial Infection) In P
Falciparum-Infected Red Blood Cells
Vtn Internalization Is Detected At The Early Trophozoite Stage
Colocalizes With Sera5 At The Schizont Stage And With Sera5 P47 At The Merozoite Surface
Alternative Antibody Names Anti-Vitronectin antibody
Anti-Vn antibody
Anti-S-Protein antibody
Anti-Serum-Spreading Factor antibody
Anti-V75 Cleaved Into - Vitronectin V65 Subunit - Vitronectin V10 Subunit - Somatomedin-B antibody
Anti-VTN antibody

Information sourced from Uniprot.org

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