• Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using VTI1B antibody (STJ29142) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using VTI1B antibody (STJ29142) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of mouse brain, using VTI1B antibody (STJ29142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-VTI1B antibody (1-210) (STJ29142)

SKU:
STJ29142

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VTI1B (1-210) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VTI1B
Gene ID: 10490
Uniprot ID: VTI1B_HUMAN
Immunogen Region: 1-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human VTI1B (NP_006361.1).
Immunogen Sequence: MASSAASSEHFEKLHEIFRG LHEDLQGVPERLLGTAGTEE KKKLIRDFDEKQQEANETLA EMEEELRYAPLSFRNPMMSK LRNYRKDLAKLHREVRSTPL TATPGGRGDMKYGIYAVENE HMNRLQSQRAMLLQGTESLN RATQSIERSHRIATETDQIG SEIIEELGEQRDQLERTKSR LVNTSENLSKSRKILRSMSR KVTTNKLLLS
Tissue Specificity Expressed in all tissues examined.
Function V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.
Protein Name Vesicle Transport Through Interaction With T-Snares Homolog 1b
Vesicle Transport V-Snare Protein Vti1-Like 1
Vti1-Rp1
Database Links Reactome: R-HSA-114608
Cellular Localisation Early Endosome Membrane
Single-Pass Type Iv Membrane Protein
Late Endosome Membrane
Lysosome Membrane
Cytoplasmic Granule
Recycling Endosome Membrane
Alternative Antibody Names Anti-Vesicle Transport Through Interaction With T-Snares Homolog 1b antibody
Anti-Vesicle Transport V-Snare Protein Vti1-Like 1 antibody
Anti-Vti1-Rp1 antibody
Anti-VTI1B antibody
Anti-VTI1 antibody
Anti-VTI1L antibody
Anti-VTI1L1 antibody
Anti-VTI2 antibody

Information sourced from Uniprot.org

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