Anti-VRK2 antibody (Internal) (STJ73321)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-VRK2 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Dog |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 60kDa band observed in nuclear lysates of cell line K562 (calculated MW of 58.1kDa according to NP_001123954.1). Recommended concentration: 1-3µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | VRK2 |
| Gene ID | 7444 |
| Uniprot ID | VRK2_HUMAN |
| Accession Number | NP_001129499.1; NP_006287.2; NP_001123954.1; NP_001123955.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | PNSQKVDSQKAATK |
| Specificity | This antibody is expected to recognize all isoforms (NP_001123952.1, NP_001123954.1, NP_001123955.1, NP_001129499.1). Reported variants represent identical protein: NP_001123953.1, NP_006287.2. |
Additional Info
| Post Translational Modifications | Isoform 1: Autophosphorylated. Isoform 2: Autophosphorylated. |
| Function | Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Down-regulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant. Isoform 2: Phosphorylates 'Thr-18' of p53/TP53, as well as histone H3. Reduces p53/TP53 ubiquitination by MDM2, promotes p53/TP53 acetylation by EP300 and thereby increases p53/TP53 stability and activity. |
| Protein Name | Serine/Threonine-Protein Kinase Vrk2Vaccinia-Related Kinase 2 |
| Database Links | Reactome: R-HSA-2980766 Q86Y07-2Reactome: R-HSA-2995383 Q86Y07-2Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013405Reactome: R-HSA-9013408Reactome: R-HSA-9013423 |
| Cellular Localisation | Isoform 1: CytoplasmEndoplasmic Reticulum MembraneSingle-Pass Type Iv Membrane ProteinMitochondrion MembraneNucleus EnvelopeIsoform 2: CytoplasmNucleus |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Vrk2 antibodyAnti-Vaccinia-Related Kinase 2 antibodyAnti-VRK2 antibody |
Information sourced from Uniprot.org