| Host: |
Rabbit |
| Applications: |
WB/IHC-P/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
VRK1 |
| Gene ID: |
7443 |
| Uniprot ID: |
VRK1_HUMAN |
| Immunogen Region: |
257-396 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 257-396 of human VRK1 (NP_003375.1). |
| Immunogen Sequence: |
GHLPWEDNLKDPKYVRDSKI RYRENIASLMDKCFPEKNKP GEIAKYMETVKLLDYTEKPL YENLRDILLQGLKAIGSKDD GKLDLSVVENGGLKAKTITK KRKKEIEESKEPGVEDTEWS NTQTEEAIQTRSRTRKRVQK |
| Tissue Specificity | Widely expressed. Highly expressed in fetal liver, testis and thymus. |
| Post Translational Modifications | Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53. Phosphorylation by PLK3 leads to induction of Golgi fragmentation during mitosis. |
| Function | Serine/threonine kinase involved in the regulation of key cellular processes including the cell cycle, nuclear condensation, transcription regulation, and DNA damage response. Controls chromatin organization and remodeling by mediating phosphorylation of histone H3 on 'Thr-4' and histone H2AX (H2aXT4ph). It also phosphorylates KAT5 in response to DNA damage, promoting KAT5 association with chromatin and histone acetyltransferase activity. Is involved in the regulation of cell cycle progression of neural progenitors, and is required for proper cortical neuronal migration. Is involved in neurite elongation and branching in motor neurons, and has an essential role in Cajal bodies assembly, acting through COIL phosphorylation and the control of coilin degradation. Involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, it is required to induce Golgi fragmentation. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates TP53BP1 and p53/TP53 on 'Thr-18', preventing the interaction between p53/TP53 and MDM2. Phosphorylates ATF2 which activates its transcriptional activity. Phosphorylates JUN. |
| Protein Name | Serine/Threonine-Protein Kinase Vrk1Vaccinia-Related Kinase 1 |
| Database Links | Reactome: R-HSA-2980766Reactome: R-HSA-2995383 |
| Cellular Localisation | NucleusCytoplasmCajal BodyEnriched On Chromatin During Mitosis |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Vrk1 antibodyAnti-Vaccinia-Related Kinase 1 antibodyAnti-VRK1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and VRK1 knockout (KO) 293T cells, using [KO Validated] VRK1 Rabbit polyclonal antibody (STJ11100814) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89750/358227/STJ11100814_1__49569.1713122553.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and VRK1 knockout (KO) 293T cells, using [KO Validated] VRK1 Rabbit polyclonal antibody (STJ11100814) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
