Anti-VPS41 antibody (Internal) (STJ70734)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-VPS41 (Internal) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave bands at approx 48kDa and 28kDa in human duodenum, kidney and placenta lysates after 1µg/ml antibody staining. Please note that currently we cannot find an |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | VPS41 |
| Gene ID | 27072 |
| Uniprot ID | VPS41_HUMAN |
| Accession Number | NP_055211.2; NP_542198.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | DHLKKDSQNKTLLK |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_055211.2 and NP_542198.2) |
Additional Info
| Function | Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act in part as a core component of the putative HOPS endosomal tethering complex is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. Involved in homotypic vesicle fusions between late endosomes and in heterotypic fusions between late endosomes and lysosomes implicated in degradation of endocytosed cargo. Required for fusion of autophagosomes with lysosomes. Links the HOPS complex to endosomal Rab7 via its association with RILP and to lysosomal membranes via its association with ARL8B, suggesting that these interactions may bring the compartments to close proximity for fusion. Involved in the direct trans-Golgi network to late endosomes transport of lysosomal membrane proteins independently of HOPS. Involved in sorting to the regulated secretory pathway presumably implicating the AP-3 adapter complex. May play a role in HOPS-independent function in the regulated secretory pathway. |
| Protein Name | Vacuolar Protein Sorting-Associated Protein 41 HomologS53 |
| Database Links | Reactome: R-HSA-9754560 |
| Cellular Localisation | Endosome MembranePeripheral Membrane ProteinLate Endosome MembraneEarly Endosome MembraneLysosome MembraneGolgi ApparatusTrans-Golgi NetworkCytoplasmic VesicleClathrin-Coated VesicleCytoplasmCytosol(Microbial Infection) Sequestrated At The Late Endosome By Sars Coronavirus-2/Sars-Cov-2 Orf3a Protein |
| Alternative Antibody Names | Anti-Vacuolar Protein Sorting-Associated Protein 41 Homolog antibodyAnti-S53 antibodyAnti-VPS41 antibody |
Information sourced from Uniprot.org