• Western blot analysis of Hela cells using VPS35 Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-VPS35 antibody (511-560 aa) (STJ98618)

SKU:
STJ98618

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Vacuolar protein sorting-associated protein 35 (511-560 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA 1:10000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VPS35
Gene ID: 55737
Uniprot ID: VPS35_HUMAN
Immunogen Region: 511-560 aa
Specificity: VPS35 Polyclonal Antibody detects endogenous levels of VPS35
Immunogen: Synthesized peptide derived from VPS35 at the amino acid range 511-560
Function Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35.however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Required for endosomal localization of WASHC2C. Mediates the association of the CSC with the WASH complex via WASHC2. Required for the endosomal localization of TBC1D5. (Microbial infection) The heterotrimeric retromer cargo-selective complex (CSC) mediates the exit of human papillomavirus from the early endosome and the delivery to the Golgi apparatus.
Protein Name Vacuolar Protein Sorting-Associated Protein 35
Hvps35
Maternal-Embryonic 3
Vesicle Protein Sorting 35
Database Links Reactome: R-HSA-3238698
Cellular Localisation Cytoplasm
Membrane
Peripheral Membrane Protein
Endosome
Early Endosome
Late Endosome
Localizes To Tubular Profiles Adjacent To Endosomes
Alternative Antibody Names Anti-Vacuolar Protein Sorting-Associated Protein 35 antibody
Anti-Hvps35 antibody
Anti-Maternal-Embryonic 3 antibody
Anti-Vesicle Protein Sorting 35 antibody
Anti-VPS35 antibody
Anti-MEM3 antibody
Anti-TCCCTA00141 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance