| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-VPS29 (1-186) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
VPS29 |
| Gene ID: |
51699 |
| Uniprot ID: |
VPS29_HUMAN |
| Immunogen Region: |
1-186 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human VPS29 (NP_476528.1). |
| Immunogen Sequence: |
MAGHRLVLVLGDLHIPHRCN SLPAKFKKLLVPGKIQHILC TGNLCTKESYDYLKTLAGDV HIVRGDFDENLNYPEQKVVT VGQFKIGLIHGHQVIPWGDM ASLALLQRQFDVDILISGHT HKFEAFEHENKFYINPGSAT GAYNALETNIIPSFVLMDIQ ASTVVTYVYQLIGDDVKVER IEYKKP |
| Tissue Specificity | Ubiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain. |
| Function | Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Acts also as component of the retriever complex. The retriever complex is a heterotrimeric complex related to retromer cargo-selective complex (CSC) and essential for retromer-independent retrieval and recycling of numerous cargos such as integrin alpha-5/beta-1 (ITGA5:ITGB1). In the endosomes, retriever complex drives the retrieval and recycling of NxxY-motif-containing cargo proteins by coupling to SNX17, a cargo essential for the homeostatic maintenance of numerous cell surface proteins associated with processes that include cell migration, cell adhesion, nutrient supply and cell signaling. The recruitment of the retriever complex to the endosomal membrane involves CCC and WASH complexes. Involved in GLUT1 endosome-to-plasma membrane trafficking.the function is dependent of association with ANKRD27. (Microbial infection) The heterotrimeric retromer cargo-selective complex (CSC) mediates the exit of human papillomavirus from the early endosome and the delivery to the Golgi apparatus. |
| Protein Name | Vacuolar Protein Sorting-Associated Protein 29Hvps29Pep11 HomologVesicle Protein Sorting 29 |
| Database Links | Reactome: R-HSA-3238698 |
| Cellular Localisation | CytoplasmMembranePeripheral Membrane ProteinEndosome MembraneEarly EndosomeLate Endosome |
| Alternative Antibody Names | Anti-Vacuolar Protein Sorting-Associated Protein 29 antibodyAnti-Hvps29 antibodyAnti-Pep11 Homolog antibodyAnti-Vesicle Protein Sorting 29 antibodyAnti-VPS29 antibodyAnti-DC15 antibodyAnti-DC7 antibodyAnti-MDS007 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
