• Immunohistochemistry analysis of paraffin-embedded human lung cancer using VPS28 Rabbit monoclonal antibody (STJ11102972) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using VPS28 antibody (STJ11102972) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-VPS28 antibody (122-221) [S2MR] (STJ11102972)

SKU:
STJ11102972

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-VPS28 (122-221) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VPS28
Gene ID: 51160
Uniprot ID: VPS28_HUMAN
Immunogen Region: 122-221
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 122-221 of human VPS28 (Q9UK41).
Immunogen Sequence: KGNLNRCIADVVSLFITVMD KLRLEIRAMDEIQPDLRELM ETMHRMSHLPPDFEGRQTVS QWLQTLSGMSASDELDDSQV RQMLFDLESAYNAFNRFLHA
Function Component of the ESCRT-I complex, a regulator of vesicular trafficking process.
Protein Name Vacuolar Protein Sorting-Associated Protein 28 Homolog
H-Vps28
Escrt-I Complex Subunit Vps28
Database Links Reactome: R-HSA-162588
Reactome: R-HSA-174490
Reactome: R-HSA-917729
Reactome: R-HSA-9610379
Reactome: R-HSA-9615710
Cellular Localisation Cell Membrane
Late Endosome Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Vacuolar Protein Sorting-Associated Protein 28 Homolog antibody
Anti-H-Vps28 antibody
Anti-Escrt-I Complex Subunit Vps28 antibody
Anti-VPS28 antibody

Information sourced from Uniprot.org

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