| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S2MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | VPS28 |
| Gene ID: | 51160 |
| Uniprot ID: | VPS28_HUMAN |
| Immunogen Region: | 122-221 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 122-221 of human VPS28 (Q9UK41). |
| Immunogen Sequence: | KGNLNRCIADVVSLFITVMD KLRLEIRAMDEIQPDLRELM ETMHRMSHLPPDFEGRQTVS QWLQTLSGMSASDELDDSQV RQMLFDLESAYNAFNRFLHA |
| Function | Component of the ESCRT-I complex, a regulator of vesicular trafficking process. |
| Protein Name | Vacuolar Protein Sorting-Associated Protein 28 HomologH-Vps28Escrt-I Complex Subunit Vps28 |
| Database Links | Reactome: R-HSA-162588Reactome: R-HSA-174490Reactome: R-HSA-917729Reactome: R-HSA-9610379Reactome: R-HSA-9615710 |
| Cellular Localisation | Cell MembraneLate Endosome MembranePeripheral Membrane Protein |
| Alternative Antibody Names | Anti-Vacuolar Protein Sorting-Associated Protein 28 Homolog antibodyAnti-H-Vps28 antibodyAnti-Escrt-I Complex Subunit Vps28 antibodyAnti-VPS28 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and UBE2S knockout (KO) 293T cells, using [KO Validated] UBE2S Rabbit polyclonal antibody (STJ11100037) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89109/357563/STJ11100037_1__21240.1713121689.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and UBE2S knockout (KO) 293T cells, using [KO Validated] UBE2S Rabbit polyclonal antibody (STJ11100037) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
