Anti-VPS25 antibody (1-176) (STJ115098)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal VPS25 (1-176) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | VPS25 |
| Gene ID | 84313 |
| Uniprot ID | VPS25_HUMAN |
| Immunogen Region | 1-176 |
| Immunogen Sequence | MAMSFEWPWQYRFPPFFTLQ PNVDTRQKQLAAWCSLVLSF CRLHKQSSMTVMEAQESPLF NNVKLQRKLPVESIQIVLEE LRKKGNLEWLDKSKSSFLIM WRRPEEWGKLIYQWVSRSGQ NNSVFTLYELTNGEDTEDEE FHGLDEATLLRALQALQQEH KAEIITVSDGRGVKFF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-176 of human VPS25 (NP_115729.1). |
Additional Info
| Tissue Specificity | Expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression are found in heart, skeletal muscle, brain and lung. |
| Function | Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses. |
| Protein Name | Vacuolar Protein-Sorting-Associated Protein 25Hvps25Dermal Papilla-Derived Protein 9Ell-Associated Protein Of 20 KdaEscrt-Ii Complex Subunit Vps25 |
| Database Links | Reactome: R-HSA-917729Reactome: R-HSA-9610379 |
| Cellular Localisation | CytoplasmEndosome MembraneNucleusNucleoplasmDistributes Diffusely Throughout The Cytoplasm And NucleoplasmBut Exhibits A Punctate Distribution On Coexpression With Chmp6 |
| Alternative Antibody Names | Anti-Vacuolar Protein-Sorting-Associated Protein 25 antibodyAnti-Hvps25 antibodyAnti-Dermal Papilla-Derived Protein 9 antibodyAnti-Ell-Associated Protein Of 20 Kda antibodyAnti-Escrt-Ii Complex Subunit Vps25 antibodyAnti-VPS25 antibodyAnti-DERP9 antibodyAnti-EAP20 antibody |
Information sourced from Uniprot.org