Anti-Vinculin antibody (STJA0038089)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human Vinculin
General Information
| Short Description | Rabbit monoclonal anti-Vinculin for use in IHC-P in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purified |
| Dilution Range | IHC 1:100-1:200 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | VCL |
| Gene ID | 7414 |
| Uniprot ID | VINC_HUMAN |
| Immunogen | A synthesized peptide derived from human Vinculin |
Additional Info
| Tissue Specificity | Metavinculin is muscle-specific. |
| Post Translational Modifications | Phosphorylated.on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques. Acetylated.mainly by myristic acid but also by a small amount of palmitic acid. |
| Function | Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. |
| Protein Name | VinculinMetavinculinMv |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-445355Reactome: R-HSA-5674135Reactome: R-HSA-6798695Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-9725370Reactome: R-HSA-9856530Reactome: R-HSA-9860927 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmic SideCell JunctionAdherens JunctionFocal AdhesionCytoplasmCytoskeletonSarcolemmaCell ProjectionPodosomeRecruitment To Cell-Cell Junctions Occurs In A Myosin Ii-Dependent MannerInteraction With Ctnnb1 Is Necessary For Its Localization To The Cell-Cell Junctions |
| Alternative Antibody Names | Anti-Vinculin antibodyAnti-Metavinculin antibodyAnti-Mv antibodyAnti-VCL antibody |
Information sourced from Uniprot.org