| Host: |
Mouse |
| Applications: |
IHC/WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Vimentin (400-466 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
ABT019 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2bk |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range: |
IHC-P 1:100-500WB 1:200-1000IF 1:100-500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
VIM |
| Gene ID: |
7431 |
| Uniprot ID: |
VIME_HUMAN |
| Immunogen Region: |
400-466 aa |
| Specificity: |
The antibody can specifically recognize human Vimentin protein. In western blotting of HEK293 cell lysate, the antibody can label a 54KDa band corresponding to Vimentin. |
| Immunogen: |
Synthesized peptide derived from the human Vimentin at the amino acid range 400-466 |
| Post Translational Modifications | Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Phosphorylated on tyrosine residues by SRMS. O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
| Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. |
| Protein Name | Vimentin |
| Database Links | Reactome: R-HSA-264870Reactome: R-HSA-390522Reactome: R-HSA-6785807Reactome: R-HSA-9013422Reactome: R-HSA-9613829Reactome: R-HSA-9615710Reactome: R-HSA-9646399 |
| Cellular Localisation | CytoplasmCytoskeletonNucleus MatrixCell Membrane |
| Alternative Antibody Names | Anti-Vimentin antibodyAnti-VIM antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
