Anti-VGluT3 antibody (N-Term) (STJ13100516)
SPECIFICATIONS
ClonalityPolyclonal
HostGuinea Pig
ConjugationUnconjugated
ImmunogenA synthetic peptide to n-terminal region of human VGluT3 conjugated to blue carrier protein was used as the antigen.
General Information
| Short Description | Guinea Pig polyclonal anti-VGluT3 (N-Term) for use in IHC and WB in Human, Rat, Mouse and Marmoset samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Guinea Pig |
| Reactivity | Human/Rat/Mouse/Marmoset |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | IHC, WB. A dilution of 1: 500 is recommended for WB and 1:250 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Slc17a8 |
| Gene ID | 216227 |
| Uniprot ID | VGLU3_MOUSE |
| Immunogen | A synthetic peptide to n-terminal region of human VGluT3 conjugated to blue carrier protein was used as the antigen. |
| Immunogen Region | N-Term |
| Specificity | Specific for VGLUT3. |
Additional Info
| Function | Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, sodium and phosphate. At the synaptic vesicle membrane, mainly functions as an uniporter that mediates the uptake of L-glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. The L-glutamate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane. In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification. At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation. The symporter activity is electrogenic. Moreover, operates synergistically with SLC18A3/VACHT under a constant H(+) gradient, thereby allowing striatal vesicular acetylcholine uptake. |
| Protein Name | Vesicular Glutamate Transporter 3Vglut3Solute Carrier Family 17 Member 8 |
| Database Links | Reactome: R-MMU-428643 |
| Cellular Localisation | Cytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneCell MembraneMulti-Pass Membrane ProteinSynapseSynaptosome |
| Alternative Antibody Names | Anti-Vesicular Glutamate Transporter 3 antibodyAnti-Vglut3 antibodyAnti-Solute Carrier Family 17 Member 8 antibodyAnti-Slc17a8 antibodyAnti-Vglut3 antibody |
Information sourced from Uniprot.org