Anti-VFP antibody (STJ140120)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
ImmunogenPurified recombinant GFP peptide produced in E. coli.
General Information
| Short Description | Goat polyclonal Venus Fluorescent Protein antibody for use in WB, IF, IHC-P, IHC-F and IEM in yfp, gfp, egfp and venus samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/IHC-P/IHC-F/IEM |
| Host | Goat |
| Reactivity | YFP/GFP/EGFP/Venus |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 3 mg/mL |
| Purification | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range | WB 1:500-1:2000IHC-F 1:50-1:1000IHC-P 1:50-1:1000IF 1:50-1:1000IEM 1:50-1:1000 |
| Formulation | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Immunogen | Purified recombinant GFP peptide produced in E. coli. |
| Specificity | In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody also recognizes Venus/GFP and does not cross-react to mCherry/red fluorescent proteins. |
Additional Info
| Background | Goat polyclonal antibody to Venus. Venus is a genetic mutant of green fluorescent protein (GFP) , derived from Aequorea victoria. Its excitation/emission peaks are 515/528 nm respectively and is often used as a donor-acceptor pair by researchers in FRET based co-localization studies. |
Information sourced from Uniprot.org