Anti-Versican antibody (3200-3396) [S2MR] (STJ11103072)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Versican (3200-3396) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S2MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IHC-P:1:200-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | VCAN |
| Gene ID | 1462 |
| Uniprot ID | CSPG2_HUMAN |
| Immunogen Region | 3200-3396 |
| Immunogen Sequence | QGAHLTSILSHEEQMFVNRV GHDYQWIGLNDKMFEHDFRW TDGSTLQYENWRPNQPDSFF SAGEDCVVIIWHENGQWNDV PCNYHLTYTCKKGTVACGQP PVVENAKTFGKMKPRYEINS LIRYHCKDGFIQRHLPTIRC LGNGRWAIPKITCMNPSAYQ RTYSMKYFKNSSSAKDNSIN TSKHDHRWSRRWQESRR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 3200-3396 of human Versican (P13611). |
Additional Info
| Tissue Specificity | Detected in placenta (at protein level). Detected in cerebrospinal fluid, fibroblasts and urine (at protein level). Expressed in the retina (at protein level). Cerebral white matter and plasma. Isoform V0: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V1: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2: Restricted to normal brain and gliomas. Isoform V3: Found in all these tissues except medulloblastomas. |
| Post Translational Modifications | Phosphorylated by FAM20C in the extracellular medium. Proteolytically cleaved by ADAMTS5 and ADAMTS15 in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration. |
| Function | May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid. |
| Protein Name | Versican Core ProteinChondroitin Sulfate Proteoglycan Core Protein 2Chondroitin Sulfate Proteoglycan 2Glial Hyaluronate-Binding ProteinGhapLarge Fibroblast ProteoglycanPg-M |
| Database Links | Reactome: R-HSA-1971475Reactome: R-HSA-2022870Reactome: R-HSA-2022923Reactome: R-HSA-2024101Reactome: R-HSA-3000178Reactome: R-HSA-3560783Reactome: R-HSA-3560801Reactome: R-HSA-3595172Reactome: R-HSA-3595174Reactome: R-HSA-3595177Reactome: R-HSA-381426Reactome: R-HSA-4420332Reactome: R-HSA-8957275 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixCell ProjectionCiliumPhotoreceptor Outer SegmentInterphotoreceptor Matrix |
| Alternative Antibody Names | Anti-Versican Core Protein antibodyAnti-Chondroitin Sulfate Proteoglycan Core Protein 2 antibodyAnti-Chondroitin Sulfate Proteoglycan 2 antibodyAnti-Glial Hyaluronate-Binding Protein antibodyAnti-Ghap antibodyAnti-Large Fibroblast Proteoglycan antibodyAnti-Pg-M antibodyAnti-VCAN antibodyAnti-CSPG2 antibody |
Information sourced from Uniprot.org