• Immunofluorescence analysis of U-2 OS cells using Versican Rabbit monoclonal antibody (STJ11103072) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using Versican Rabbit monoclonal antibody (STJ11103072) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Versican Rabbit monoclonal antibody (STJ11103072) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Versican in paraffin-embedded rat kidney using Versican Rabbit monoclonal antibody (STJ11103072) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Versican in paraffin-embedded mouse brain using Versican Rabbit monoclonal antibody (STJ11103072) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Versican in paraffin-embedded human esophageal cancer using Versican Rabbit monoclonal antibody (STJ11103072) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Versican Rabbit monoclonal antibody (STJ11103072) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Versican antibody (3200-3396) [S2MR] (STJ11103072)

SKU:
STJ11103072

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Versican (3200-3396) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VCAN
Gene ID: 1462
Uniprot ID: CSPG2_HUMAN
Immunogen Region: 3200-3396
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 3200-3396 of human Versican (P13611).
Immunogen Sequence: QGAHLTSILSHEEQMFVNRV GHDYQWIGLNDKMFEHDFRW TDGSTLQYENWRPNQPDSFF SAGEDCVVIIWHENGQWNDV PCNYHLTYTCKKGTVACGQP PVVENAKTFGKMKPRYEINS LIRYHCKDGFIQRHLPTIRC LGNGRWAIPKITCMNPSAYQ RTYSMKYFKNSSSAKDNSIN TSKHDHRWSRRWQESRR
Tissue Specificity Detected in placenta (at protein level). Detected in cerebrospinal fluid, fibroblasts and urine (at protein level). Expressed in the retina (at protein level). Cerebral white matter and plasma. Isoform V0: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V1: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2: Restricted to normal brain and gliomas. Isoform V3: Found in all these tissues except medulloblastomas.
Post Translational Modifications Phosphorylated by FAM20C in the extracellular medium. Proteolytically cleaved by ADAMTS5 and ADAMTS15 in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration.
Function May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
Protein Name Versican Core Protein
Chondroitin Sulfate Proteoglycan Core Protein 2
Chondroitin Sulfate Proteoglycan 2
Glial Hyaluronate-Binding Protein
Ghap
Large Fibroblast Proteoglycan
Pg-M
Database Links Reactome: R-HSA-1971475
Reactome: R-HSA-2022870
Reactome: R-HSA-2022923
Reactome: R-HSA-2024101
Reactome: R-HSA-3000178
Reactome: R-HSA-3560783
Reactome: R-HSA-3560801
Reactome: R-HSA-3595172
Reactome: R-HSA-3595174
Reactome: R-HSA-3595177
Reactome: R-HSA-381426
Reactome: R-HSA-4420332
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Cell Projection
Cilium
Photoreceptor Outer Segment
Interphotoreceptor Matrix
Alternative Antibody Names Anti-Versican Core Protein antibody
Anti-Chondroitin Sulfate Proteoglycan Core Protein 2 antibody
Anti-Chondroitin Sulfate Proteoglycan 2 antibody
Anti-Glial Hyaluronate-Binding Protein antibody
Anti-Ghap antibody
Anti-Large Fibroblast Proteoglycan antibody
Anti-Pg-M antibody
Anti-VCAN antibody
Anti-CSPG2 antibody

Information sourced from Uniprot.org

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