• Western blot analysis of extracts of various cell lines, using VEPH1 antibody (STJ110808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of Mouse brain, using VEPH1 antibody (STJ110808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.
  • Western blot analysis of extracts of mouse brain, using VEPH1 antibody (STJ110808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.
  • Western blot analysis of extracts of various cell lines, using VEPH1 antibody (STJ110808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-VEPH1 antibody (448-547) (STJ110808)

SKU:
STJ110808

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VEPH1 (448-547) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VEPH1
Gene ID: 79674
Uniprot ID: MELT_HUMAN
Immunogen Region: 448-547
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 448-547 of human VEPH1 (NP_078897.2).
Immunogen Sequence: KSLAFHTMLTKGVGSDDGED ENRGDIPASISLSEIDPLGQ GNDKLPFKTDTERSQLGESS VSYPNIIHIDSENLSETVKE NSQEETPETTASPIEYQDKL
Function Interacts with TGF-beta receptor type-1 (TGFBR1) and inhibits dissociation of activated SMAD2 from TGFBR1, impeding its nuclear accumulation and resulting in impaired TGF-beta signaling. May also affect FOXO, Hippo and Wnt signaling.
Protein Name Ventricular Zone-Expressed Ph Domain-Containing Protein Homolog 1
Protein Melted
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Ventricular Zone-Expressed Ph Domain-Containing Protein Homolog 1 antibody
Anti-Protein Melted antibody
Anti-VEPH1 antibody
Anti-KIAA1692 antibody
Anti-VEPH antibody

Information sourced from Uniprot.org

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