• Western blot analysis of extracts of various cell lines, using VDR antibody (STJ113337) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of HeLa cells using VDR rabbit polyclonal antibody (STJ113337) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using VDR rabbit polyclonal antibody (STJ113337) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using VDR rabbit polyclonal antibody (STJ113337) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-VDR antibody (1-300) (STJ113337)

SKU:
STJ113337

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VDR (1-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VDR
Gene ID: 7421
Uniprot ID: VDR_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human VDR (NP_000367.1).
Immunogen Sequence: MEAMAASTSLPDPGDFDRNV PRICGVCGDRATGFHFNAMT CEGCKGFFRRSMKRKALFTC PFNGDCRITKDNRRHCQACR LKRCVDIGMMKEFILTDEEV QRKREMILKRKEEEALKDSL RPKLSEEQQRIIAILLDAHH KTYDPTYSDFCQFRPPVRVN DGGGSHPSRPNSRHTPSFSG DSSSSCSDHCITSSDMMDSS SFSNLDLSEEDSDDPSVTLE LSQLSMLPHLADLVSYSIQ
Function Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Plays a central role in calcium homeostasis. Also functions as a receptor for the secondary bile acid lithocholic acid (LCA) and its metabolites.
Protein Name Vitamin D3 Receptor
Vdr
1 -25-Dihydroxyvitamin D3 Receptor
Nuclear Receptor Subfamily 1 Group I Member 1
Database Links Reactome: R-HSA-196791
Reactome: R-HSA-383280
Reactome: R-HSA-4090294
Cellular Localisation Nucleus
Cytoplasm
Localizes Mainly To The Nucleus
Translocated Into The Nucleus Via Both Ligand-Dependent And Ligand-Independent Pathways
Ligand-Independent Nuclear Translocation Is Mediated By Ipo4
Alternative Antibody Names Anti-Vitamin D3 Receptor antibody
Anti-Vdr antibody
Anti-1 -25-Dihydroxyvitamin D3 Receptor antibody
Anti-Nuclear Receptor Subfamily 1 Group I Member 1 antibody
Anti-VDR antibody
Anti-NR1I1 antibody

Information sourced from Uniprot.org

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