| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | VCL |
| Gene ID: | 7414 |
| Uniprot ID: | VINC_HUMAN |
| Immunogen Region: | 751-850 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 751-850 of human Vinculin (NP_054706.1). |
| Immunogen Sequence: | AGATSIARRANRILLVAKRE VENSEDPKFREAVKAASDEL SKTISPMVMDAKAVAGNISD PGLQKSFLDSGYRILGAVAK VREAFQPQEPDFPPPPPDLE |
| Tissue Specificity | Metavinculin is muscle-specific. |
| Post Translational Modifications | Phosphorylated.on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques. Acetylated.mainly by myristic acid but also by a small amount of palmitic acid. |
| Function | Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. |
| Protein Name | Vinculin Metavinculin Mv |
| Database Links | Reactome: R-HSA-114608 Reactome: R-HSA-445355 Reactome: R-HSA-5674135 Reactome: R-HSA-6798695 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-9649948 Reactome: R-HSA-9656223 Reactome: R-HSA-9725370 |
| Cellular Localisation | Cell Membrane Peripheral Membrane Protein Cytoplasmic Side Cell Junction Adherens Junction Focal Adhesion Cytoplasm Cytoskeleton Sarcolemma Cell Projection Podosome Recruitment To Cell-Cell Junctions Occurs In A Myosin Ii-Dependent Manner Interaction With Ctnnb1 Is Necessary For Its Localization To The Cell-Cell Junctions |
| Alternative Antibody Names | Anti-Vinculin antibody Anti-Metavinculin antibody Anti-Mv antibody Anti-VCL antibody |
Information sourced from Uniprot.org