Anti-VCL antibody (100-200) [PT0372] (STJA0006223)

SKU:
STJA0006223

Current Stock:
Host: Mouse
Applications: IHC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Vinculin (100-200) is suitable for use in Immunohistochemistry and ELISA research applications.
Clonality: Monoclonal
Clone ID: PT0372
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was purified using affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-p 1:100-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VCL
Gene ID: 7414
Uniprot ID: VINC_HUMAN
Immunogen Region: 100-200
Specificity: Vinculin Antibody detects endogenous levels of Vinculin protein.
Immunogen: Synthesized peptide derived from human Vinculin. AA range:786-835 AA range: 100-200
Tissue Specificity Metavinculin is muscle-specific.
Post Translational Modifications Phosphorylated.on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques. Acetylated.mainly by myristic acid but also by a small amount of palmitic acid.
Function Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Protein Name Vinculin
Metavinculin
Mv
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-445355
Reactome: R-HSA-5674135
Reactome: R-HSA-6798695
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9725370
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Junction
Adherens Junction
Focal Adhesion
Cytoplasm
Cytoskeleton
Sarcolemma
Cell Projection
Podosome
Recruitment To Cell-Cell Junctions Occurs In A Myosin Ii-Dependent Manner
Interaction With Ctnnb1 Is Necessary For Its Localization To The Cell-Cell Junctions
Alternative Antibody Names Anti-Vinculin antibody
Anti-Metavinculin antibody
Anti-Mv antibody
Anti-VCL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance