| Short Description : | Goat polyclonal antibody anti-VAV2 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications: | Pep-ELISA/WB/IHC |
| Host: | Goat |
| Reactivity: | Human/Mouse/Rat/Dog/Pig |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 0.5 mg/mL |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 100kDa band observed in 293 lysate (predicted MW of 100kDa according to NP_003362). Recommended for use at 1-1.5µg/ml.IHC: Paraffin embedded Human Colon. Recommended concen |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | VAV2 |
| Gene ID: | 7410 |
| Uniprot ID: | VAV2_HUMAN |
| Accession Number: | NP_001127870.1; NP_003362.2 |
| Immunogen Region: | Internal |
| Immunogen Sequence: | ETEAKYYRTLEDIEC |
| Specificity: | This antobody is expected to recognise both reported isoforms (NP_001127870.1 and NP_003362.2). |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and VAV2 knockout (KO) HeLa cells, using [KO Validated] VAV2 Rabbit polyclonal antibody (STJ11100759) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89696/358181/STJ11100759_1__51568.1713122492.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and VAV2 knockout (KO) HeLa cells, using [KO Validated] VAV2 Rabbit polyclonal antibody (STJ11100759) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
