| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | VAV2 |
| Gene ID: | 7410 |
| Uniprot ID: | VAV2_HUMAN |
| Immunogen Region: | 570-700 |
| Immunogen Sequence: | PPCKFTSPADLDASGAGPGP KMVAMQNYHGNPAPPGKPVL TFQTGDVLELLRGDPESPWW EGRLVQTRKSGYFPSSSVKP CPVDGRPPISRPPSREIDYT AYPWFAGNMERQQTDNLLKS HASGTYLIRER |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 570-700 of human VAV2 (NP_001127870.1). |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Phosphorylated on tyrosine residues in response to FGR activation. |
| Function | Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. |
| Protein Name | Guanine Nucleotide Exchange Factor Vav2Vav-2 |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-193648Reactome: R-HSA-2029482Reactome: R-HSA-2424491Reactome: R-HSA-2871796Reactome: R-HSA-2871809Reactome: R-HSA-3928665Reactome: R-HSA-416482Reactome: R-HSA-4420097Reactome: R-HSA-445144Reactome: R-HSA-5218920Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013408Reactome: R-HSA-9013423Reactome: R-HSA-9664422Reactome: R-HSA-9748787 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Guanine Nucleotide Exchange Factor Vav2 antibodyAnti-Vav-2 antibodyAnti-VAV2 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and VAV2 knockout (KO) HeLa cells, using [KO Validated] VAV2 Rabbit polyclonal antibody (STJ11100759) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89696/358181/STJ11100759_1__51568.1713122492.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and VAV2 knockout (KO) HeLa cells, using [KO Validated] VAV2 Rabbit polyclonal antibody (STJ11100759) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
