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Anti-VAV2 antibody (100-200) [S6MR] (STJ11101896)

Immunohistochemistry analysis of paraffin-embedded human appendix using VAV2 Rabbit monoclonal antibody (STJ11101896) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
Western blot analysis of extracts of various cell lines, using VAV2 Rabbit monoclonal antibody (STJ11101896) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using VAV2 Rabbit monoclonal antibody (STJ11101896) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-VAV2 antibody (100-200) [S6MR] (STJ11101896)

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STJ11101896

€183.13 - €799.38

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC
Reactivity:	Human/Mouse
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit monoclonal antibody anti-VAV2 (100-200) is suitable for use in Western Blot and Immunohistochemistry research applications.

Clonality:	Monoclonal
Clone ID:	S6MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IHC-P 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	VAV2
Gene ID:	7410
Uniprot ID:	VAV2_HUMAN
Immunogen Region:	100-200
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100-200 of human VAV2 (P52735).
Immunogen Sequence:	FDVRDFGKVISAVSRLSLHS IAQNKGIRPFPSEETTENDD DVYRSLEELADEHDLGEDIY DCVPCEDGGDDIYEDIIKVE VQQPMIRYMQKMGMTEDDKR N

Tissue Specificity	Widely expressed.
Post Translational Modifications	Phosphorylated on tyrosine residues in response to FGR activation.
Function	Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
Protein Name	Guanine Nucleotide Exchange Factor Vav2 Vav-2
Database Links	Reactome: R-HSA-114604 Reactome: R-HSA-193648 Reactome: R-HSA-2029482 Reactome: R-HSA-2424491 Reactome: R-HSA-2871796 Reactome: R-HSA-2871809 Reactome: R-HSA-3928665 Reactome: R-HSA-416482 Reactome: R-HSA-4420097 Reactome: R-HSA-445144 Reactome: R-HSA-5218920 Reactome: R-HSA-8980692 Reactome: R-HSA-9013026 Reactome: R-HSA-9013106 Reactome: R-HSA-9013148 Reactome: R-HSA-9013149 Reactome: R-HSA-9013404 Reactome: R-HSA-9013408 Reactome: R-HSA-9013423 Reactome: R-HSA-9664422 Reactome: R-HSA-9748787
Alternative Antibody Names	Anti-Guanine Nucleotide Exchange Factor Vav2 antibody Anti-Vav-2 antibody Anti-VAV2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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