Anti-VAPB antibody (10-243) (STJ27316)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal VAPB (10-243) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | VAPB |
| Gene ID | 9217 |
| Uniprot ID | VAPB_HUMAN |
| Immunogen Region | 10-243 |
| Immunogen Sequence | LEPQHELKFRGPFTDVVTTN LKLGNPTDRNVCFKVKTTAP RRYCVRPNSGIIDAGASINV SVMLQPFDYDPNEKSKHKFM VQSMFAPTDTSDMEAVWKEA KPEDLMDSKLRCVFELPAEN DKPHDVEINKIISTTASKTE TPIVSKSLSSSLDDTEVKKV MEECKRLQGEVQRLREENKQ FKEEDGLRMRKTVQSNSPIS ALAPTGKEEGLST |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 10-222 of human VAPB (NP_004729.1). |
Additional Info
| Tissue Specificity | Ubiquitous. Isoform 1 predominates. |
| Function | Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44. Interacts with STARD3 in a FFAT motif phosphorylation dependent manner. Via interaction with WDR44 participates in neosynthesized protein export. Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation. |
| Protein Name | Vesicle-Associated Membrane Protein-Associated Protein B/CVamp-B/Vamp-CVamp-Associated Protein B/CVap-B/Vap-C |
| Database Links | Reactome: R-HSA-1660661Reactome: R-HSA-8980692Reactome: R-HSA-9013106Reactome: R-HSA-9013404Reactome: R-HSA-9013405Reactome: R-HSA-9013408 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Iv Membrane ProteinPresent In Mitochondria-Associated Membranes That Are Endoplasmic Reticulum Membrane Regions Closely Apposed To The Outer Mitochondrial Membrane |
| Alternative Antibody Names | Anti-Vesicle-Associated Membrane Protein-Associated Protein B/C antibodyAnti-Vamp-B/Vamp-C antibodyAnti-Vamp-Associated Protein B/C antibodyAnti-Vap-B/Vap-C antibodyAnti-VAPB antibodyAnti-UNQ484 antibodyAnti-PRO983 antibody |
Information sourced from Uniprot.org