• Immunofluorescence analysis of U2OS cells using VAPB Rabbit polyclonal antibody (STJ27316) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using VAPB Rabbit polyclonal antibody (STJ27316) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using VAPB Rabbit polyclonal antibody (STJ27316) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using VAPB antibody (STJ27316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-VAPB antibody (10-243) (STJ27316)

SKU:
STJ27316

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VAPB (10-243) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VAPB
Gene ID: 9217
Uniprot ID: VAPB_HUMAN
Immunogen Region: 10-243
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 10-243 of human VAPB (NP_004729.1).
Immunogen Sequence: LEPQHELKFRGPFTDVVTTN LKLGNPTDRNVCFKVKTTAP RRYCVRPNSGIIDAGASINV SVMLQPFDYDPNEKSKHKFM VQSMFAPTDTSDMEAVWKEA KPEDLMDSKLRCVFELPAEN DKPHDVEINKIISTTASKTE TPIVSKSLSSSLDDTEVKKV MEECKRLQGEVQRLREENKQ FKEEDGLRMRKTVQSNSPIS ALAPTGKEEGLST
Tissue Specificity Ubiquitous. Isoform 1 predominates.
Function Endoplasmic reticulum-anchored protein that mediates the formation of contact sites between the endoplasmic (ER) and late endosomes via interaction with STARD3 in a FFAT motif phosphorylation dependent manner. Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation.
Protein Name Vesicle-Associated Membrane Protein-Associated Protein B/C
Vamp-B/Vamp-C
Vamp-Associated Protein B/C
Vap-B/Vap-C
Database Links Reactome: R-HSA-1660661
Reactome: R-HSA-8980692
Reactome: R-HSA-9013106
Reactome: R-HSA-9013404
Reactome: R-HSA-9013405
Reactome: R-HSA-9013408
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Iv Membrane Protein
Present In Mitochondria-Associated Membranes That Are Endoplasmic Reticulum Membrane Regions Closely Apposed To The Outer Mitochondrial Membrane
Alternative Antibody Names Anti-Vesicle-Associated Membrane Protein-Associated Protein B/C antibody
Anti-Vamp-B/Vamp-C antibody
Anti-Vamp-Associated Protein B/C antibody
Anti-Vap-B/Vap-C antibody
Anti-VAPB antibody
Anti-UNQ484 antibody
Anti-PRO983 antibody

Information sourced from Uniprot.org

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