| Host: |
NZ White Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Nz White Rabbit polyclonal antibody anti-VACHT is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Shipped as lyophilised. Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material. |
| Purification: |
Ammonium sulphate precipitation |
| Dilution Range: |
A working concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
SLC18A3 |
| Gene ID: |
6572 |
| Uniprot ID: |
VACHT_HUMAN |
| Specificity: |
Specific for VACHT. |
| Immunogen: |
A synthetic peptide from human VACHT conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and mouse. |
| Function | Electrogenic antiporter that exchanges one cholinergic neurotransmitter, acetylcholine or choline, with two intravesicular protons across the membrane of synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to store neurotransmitters inside the vesicles prior to their release via exocytosis. Determines cholinergic vesicular quantal size at presynaptic nerve terminals in developing neuro-muscular junctions with an impact on motor neuron differentiation and innervation pattern. Part of forebrain cholinergic system, regulates hippocampal synapse transmissions that underlie spatial memory formation. Can transport serotonin. |
| Protein Name | Vesicular Acetylcholine TransporterVachtSolute Carrier Family 18 Member 3 |
| Database Links | Reactome: R-HSA-264642Reactome: R-HSA-8856825Reactome: R-HSA-8856828 |
| Cellular Localisation | Cytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Vesicular Acetylcholine Transporter antibodyAnti-Vacht antibodyAnti-Solute Carrier Family 18 Member 3 antibodyAnti-SLC18A3 antibodyAnti-VACHT antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
