Anti-UXT, Biotinylated antibody (C-Term) {Biotin} (STJ73205)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationBiotin
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-UXT, Biotinylated (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 20kDa band observed in Human Kidney lysates (calculated MW of 19.6kDa according to NP_705582.1). See non-biotinylated parental product's datasheet for further QC data. Recommend |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | UXT |
| Gene ID | 8409 |
| Uniprot ID | UXT_HUMAN |
| Accession Number | NP_705582.1; NP_004173.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | DHRDKVYEQLAKY. |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_004173.1; NP_705582.1). |
Additional Info
| Post Translational Modifications | Isoform 2: Ubiquitinated by E3 ubiquitin-protein ligase complex containing FBXO7.leading to proteasomal degradation. |
| Function | Involved in gene transcription regulation. Acts in concert with the corepressor URI1 to regulate androgen receptor AR-mediated transcription. Together with URI1, associates with chromatin to the NKX3-1 promoter region. Negatively regulates the transcriptional activity of the estrogen receptor ESR1 by inducing its translocation into the cytoplasm. May act as nuclear chaperone that facilitates the formation of the NF-kappa-B enhanceosome and thus positively regulates NF-kappa-B transcription activity. Potential component of mitochondrial-associated LRPPRC, a multidomain organizer that potentially integrates mitochondria and the microtubular cytoskeleton with chromosome remodeling. Increasing concentrations of UXT contributes to progressive aggregation of mitochondria and cell death potentially through its association with LRPPRC. Suppresses cell transformation and it might mediate this function by interaction and inhibition of the biological activity of cell proliferation and survival stimulatory factors like MECOM. Isoform 1: Plays a role in protecting cells against TNF-alpha-induced apoptosis by preventing the recruitment of FADD and caspase 8 to the apoptotic complex I, composed of TRADD, TRAF2 and RIPK1/RIP. |
| Protein Name | Protein UxtAndrogen Receptor Trapped Clone 27 ProteinArt-27Ubiquitously Expressed Transcript Protein |
| Database Links | Reactome: R-HSA-8953750 |
| Cellular Localisation | Isoform 1: CytoplasmIsoform 2: NucleusCytoplasmNucleusCytoskeletonMicrotubule Organizing CenterCentrosomeSpindle PolePredominantly Localizes To The NucleusLocalizes To Spindle Pole During Mitosis |
| Alternative Antibody Names | Anti-Protein Uxt antibodyAnti-Androgen Receptor Trapped Clone 27 Protein antibodyAnti-Art-27 antibodyAnti-Ubiquitously Expressed Transcript Protein antibodyAnti-UXT antibodyAnti-HSPC024 antibody |
Information sourced from Uniprot.org