Anti-USP8 antibody (1-270) (STJ29111)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal USP8 (1-270) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | USP8 |
| Gene ID | 9101 |
| Uniprot ID | UBP8_HUMAN |
| Immunogen Region | 1-270 |
| Immunogen Sequence | MPAVASVPKELYLSSSLKDL NKKTEVKPEKISTKSYVHSA LKIFKTAEECRLDRDEERAY VLYMKYVTVYNLIKKRPDFK QQQDYFHSILGPGNIKKAVE EAERLSESLKLRYEEAEVRK KLEEKDRQEEAQRLQQKRQE TGREDGGTLAKGSLENVLDS KDKTQKSNGEKNEKCETKEK GAITAKELYTMMTDKNISLI IMDARRMQDYQDSCILHSLS VPEEAISPGVTASWIEAHL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human USP8 (NP_005145.3). |
Additional Info
| Post Translational Modifications | Phosphorylation of Ser-718 is essential for interaction with YWHAE and for cytosol localization. Undergoes dephosphorylation at Ser-718 in the M phase. Tyrosine-phosphorylated in its N-terminal half in an EGFR-dependent manner. Ubiquitinated. Inactive form is mostly monoubiquitinated, but polyubiquitination happens too. Ubiquitination is increased in EGF-stimulated cells. Ubiquitination of active form is undetectable, suggesting a possibility that USP8 deubiquitinates itself, thereby regulating its own function. |
| Function | Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controls tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1. Deubiquitinates BACE1 which inhibits BACE1 lysosomal degradation and modulates BACE-mediated APP cleavage and amyloid-beta formation. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 8Deubiquitinating Enzyme 8Ubiquitin Isopeptidase YHubpyUbiquitin Thioesterase 8Ubiquitin-Specific-Processing Protease 8 |
| Database Links | Reactome: R-HSA-1358803Reactome: R-HSA-4641263Reactome: R-HSA-5689880Reactome: R-HSA-6807004 |
| Cellular Localisation | CytoplasmNucleusEndosome MembranePeripheral Membrane ProteinCell Membrane |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 8 antibodyAnti-Deubiquitinating Enzyme 8 antibodyAnti-Ubiquitin Isopeptidase Y antibodyAnti-Hubpy antibodyAnti-Ubiquitin Thioesterase 8 antibodyAnti-Ubiquitin-Specific-Processing Protease 8 antibodyAnti-USP8 antibodyAnti-KIAA0055 antibodyAnti-UBPY antibody |
Information sourced from Uniprot.org