| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | USP5 |
| Gene ID: | 8078 |
| Uniprot ID: | UBP5_HUMAN |
| Immunogen Region: | 1-280 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human USP5 (NP_003472.2). |
| Immunogen Sequence: | MAELSEEALLSVLPTIRVPK AGDRVHKDECAFSFDTPESE GGLYICMNTFLGFGKQYVER HFNKTGQRVYLHLRRTRRPK EEDPATGTGDPPRKKPTRLA IGVEGGFDLSEEKFELDEDV KIVILPDYLEIARDGLGGLP DIVRDRVTSAVEALLSADSA SRKQEVQAWDGEVRQVSKHA FSLKQLDNPARIPPCGWKCS KCDMRENLWLNLTDGSILCG RRYFDGSGGNNHAVEHYRE |
| Function | Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 5Deubiquitinating Enzyme 5Isopeptidase TUbiquitin Thioesterase 5Ubiquitin-Specific-Processing Protease 5 |
| Database Links | Reactome: R-HSA-5689880Reactome: R-HSA-8866652 |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 5 antibodyAnti-Deubiquitinating Enzyme 5 antibodyAnti-Isopeptidase T antibodyAnti-Ubiquitin Thioesterase 5 antibodyAnti-Ubiquitin-Specific-Processing Protease 5 antibodyAnti-USP5 antibodyAnti-ISOT antibody |
Information sourced from Uniprot.org