Anti-USP37 antibody (150-250) (STJ118042)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal USP37 (150-250) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | USP37 |
| Gene ID | 57695 |
| Uniprot ID | UBP37_HUMAN |
| Immunogen Region | 150-250 |
| Immunogen Sequence | SLETKDDIPFRKVLGNPGRG SIKTVAGSGIARTIPSLTST STPLRSGLLENRTEKRKRMI STGSELNEDYPKENDSSSNN KAMTDPSRKYLTSSREKQLS L |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human USP37 (NP_065986.2). |
Additional Info
| Tissue Specificity | Expressed in brain and prostate. |
| Post Translational Modifications | Polyubiquitinated via 'Lys-11'-linked ubiquitin by the APC(CDH1) complex during late mitosis, leading to its degradation. Able to mediate auto-deubiquitination. Phosphorylated at Ser-628 by CDK2 during G1/S phase but not during mitosis.phosphorylation at Ser-628 is required for deubiquitinase activity. Also polyubiquitinated during early G1 phase, without leading to degradation. Phosphorylated at Ser-114 by ATM following DNA damage, which in turn increases its deubiquitination activity towards BLM. |
| Function | Deubiquitinase that plays a role in different processes including cell cycle regulation, DNA replication or DNA damage response. Antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Phosphorylation at Ser-628 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2). Plays an important role in the regulation of DNA replication by stabilizing the licensing factor CDT1. Also plays an essential role beyond S-phase entry to promote the efficiency and fidelity of replication by deubiquitinating checkpoint kinase 1/CHK1, promoting its stability. Sustains the DNA damage response (DDR) by deubiquitinating and stabilizing the ATP-dependent DNA helicase BLM. Mechanistically, DNA double-strand breaks (DSB) promotes ATM-mediated phosphorylation of USP37 and enhances the binding between USP37 and BLM. Promotes cell migration by deubiquitinating and stabilizing the epithelial-mesenchymal transition (EMT)-inducing transcription factor SNAI. Plays a role in the regulation of mitotic spindle assembly and mitotic progression by associating with chromatin-associated WAPL and stabilizing it through deubiquitination. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 37Deubiquitinating Enzyme 37Ubiquitin Thioesterase 37Ubiquitin-Specific-Processing Protease 37 |
| Database Links | Reactome: R-HSA-5689880 |
| Cellular Localisation | NucleusChromosome |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 37 antibodyAnti-Deubiquitinating Enzyme 37 antibodyAnti-Ubiquitin Thioesterase 37 antibodyAnti-Ubiquitin-Specific-Processing Protease 37 antibodyAnti-USP37 antibodyAnti-KIAA1594 antibody |
Information sourced from Uniprot.org