Anti-USP3 antibody (STJ191574)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from part of the human protein
General Information
| Short Description | Rabbit polyclonal anti-Ubiquitin carboxyl-terminal hydrolase 3 for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | USP3 |
| Gene ID | 9960 |
| Uniprot ID | UBP3_HUMAN |
| Immunogen | Synthesized peptide derived from part of the human protein |
| Specificity | UBP3 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Function | Deubiquitinase that plays a role in several cellular processes including transcriptional regulation, cell cycle progression or innate immunity. In response to DNA damage, deubiquitinates monoubiquitinated target proteins such as histone H2A and H2AX and thereby counteracts RNF168- and RNF8-mediated ubiquitination. In turn, participates in the recruitment of DNA damage repair factors to DNA break sites. Required for proper progression through S phase and subsequent mitotic entry. Acts as a positive regulator of TP53 by deubiquitinating and stabilizing it to promote normal cell proliferation and transformation. Participates in establishing tolerance innate immune memory through non-transcriptional feedback. Mechanistically, negatively regulates TLR-induced NF-kappa-B signaling by targeting and removing the 'Lys-63'-linked polyubiquitin chains on MYD88. Negatively regulates the activation of type I interferon signaling by mediating 'Lys-63'-linked polyubiquitin chains on RIGI and IFIH1. Also deubiquinates ASC/PYCARD, the central adapter mediating the assembly and activation of most inflammasomes, and thereby promotes inflammasome activation. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 3Deubiquitinating Enzyme 3Ubiquitin Thioesterase 3Ubiquitin-Specific-Processing Protease 3 |
| Database Links | Reactome: R-HSA-5689880 |
| Cellular Localisation | NucleusCytoplasmLocalizes Preferentially With Monoubiquitinated H2a To ChromatinUpon Nf-Kappa-B Signaling ActivationExits The Nucleus |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 3 antibodyAnti-Deubiquitinating Enzyme 3 antibodyAnti-Ubiquitin Thioesterase 3 antibodyAnti-Ubiquitin-Specific-Processing Protease 3 antibodyAnti-USP3 antibody |
Information sourced from Uniprot.org