• Western blot analysis of various lysates, using USP25 antibody (STJ114462) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-USP25 antibody (1-230) (STJ114462)

SKU:
STJ114462

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-USP25 (1-230) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP25
Gene ID: 29761
Uniprot ID: UBP25_HUMAN
Immunogen Region: 1-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human USP25 (NP_037528.3).
Immunogen Sequence: MTVEQNVLQQSAAQKHQQTF LNQLREITGINDTQILQQAL KDSNGNLELAVAFLTAKNAK TPQQEETTYYQTALPGNDRY ISVGSQADTNVIDLTGDDKD DLQRAIALSLAESNRAFRET GITDEEQAISRVLEASIAEN KACLKRTPTEVWRDSRNPYD RKRQDKAPVGLKNVGNTCWF SAVIQSLFNLLEFRRLVLNY KPPSNAQDLPRNQKEHRNLP FMRELRYLFA
Tissue Specificity Isoform USP25a is found in most adult and fetal tissues.expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific.
Post Translational Modifications Acetylated. Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially with SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue. Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions. Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.
Function Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains. The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 25
Deubiquitinating Enzyme 25
Usp On Chromosome 21
Ubiquitin Thioesterase 25
Ubiquitin-Specific-Processing Protease 25
Database Links Reactome: R-HSA-5689880
Cellular Localisation Cytoplasm
Isoform Usp25m: Cytoplasm
Nucleus
Some Transient Punctuate Nuclear Location In Myotubes During Myocyte Development
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase 25 antibody
Anti-Deubiquitinating Enzyme 25 antibody
Anti-Usp On Chromosome 21 antibody
Anti-Ubiquitin Thioesterase 25 antibody
Anti-Ubiquitin-Specific-Processing Protease 25 antibody
Anti-USP25 antibody
Anti-USP21 antibody

Information sourced from Uniprot.org

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