Anti-USP16/UBP-M antibody (N-Term) (STJ73284)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-USP16/UBP-M (N-Term) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Kidney shows textured cytoplasm staining in the convoluted tubules. Recommended concentration, 4-6µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | USP16 |
| Gene ID | 10600 |
| Uniprot ID | UBP16_HUMAN |
| Accession Number | NP_006438.1; NP_001001992.1 |
| Immunogen Region | N-Term |
| Immunogen Sequence | GKKRTKGKTVPIDD |
| Specificity | This antibody is expected to recognise isoform a (NP_006438.1) and isoform b (NP_001001992.1). Reported variants represent identical protein (NP_006438.1; NP_001027582.1). |
Additional Info
| Post Translational Modifications | Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity. |
| Function | Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B. Also deubiquitinates non-histone proteins, such as ribosomal protein RPS27A: deubiquitination of monoubiquitinated RPS27A promotes maturation of the 40S ribosomal subunit. Also mediates deubiquitination of tektin proteins (TEKT1, TEKT2, TEK3, TEKT4 and TEKT5), promoting their stability. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 16Deubiquitinating Enzyme 16Ubiquitin Thioesterase 16Ubiquitin-Processing Protease Ubp-MUbiquitin-Specific-Processing Protease 16 |
| Database Links | Reactome: R-HSA-5689880 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 16 antibodyAnti-Deubiquitinating Enzyme 16 antibodyAnti-Ubiquitin Thioesterase 16 antibodyAnti-Ubiquitin-Processing Protease Ubp-M antibodyAnti-Ubiquitin-Specific-Processing Protease 16 antibodyAnti-USP16 antibodyAnti-MSTP039 antibody |
Information sourced from Uniprot.org