| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat/Monkey |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-USP16 (1-280) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
USP16 |
| Gene ID: |
10600 |
| Uniprot ID: |
UBP16_HUMAN |
| Immunogen Region: |
1-280 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human USP16 (NP_001001992.1). |
| Immunogen Sequence: |
MGKKRTKGKTVPIDDSSETL EPVCRHIRKGLEQGNLKKAL VNVEWNICQDCKTDNKVKDK AEEETEEKPSVWLCLKCGHQ GCGRNSQEQHALKHYLTPRS EPHCLVLSLDNWSVWCYVCD NEVQYCSSNQLGQVVDYVRK QASITTPKPEKDNGNIELEN KKLEKESKNEQEREKKENMA KENPPMNSPCQITVKGLSNL GNTCFFNAVMQNLSQTPVLR ELLKEVKMSGTIVKIEPPD |
| Tissue Specificity | Present in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
| Post Translational Modifications | Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity. |
| Function | Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B. Also deubiquitinates non-histone proteins, such as ribosomal protein RPS27A: deubiquitination of monoubiquitinated RPS27A promotes maturation of the 40S ribosomal subunit. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 16Deubiquitinating Enzyme 16Ubiquitin Thioesterase 16Ubiquitin-Processing Protease Ubp-MUbiquitin-Specific-Processing Protease 16 |
| Database Links | Reactome: R-HSA-5689880 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 16 antibodyAnti-Deubiquitinating Enzyme 16 antibodyAnti-Ubiquitin Thioesterase 16 antibodyAnti-Ubiquitin-Processing Protease Ubp-M antibodyAnti-Ubiquitin-Specific-Processing Protease 16 antibodyAnti-USP16 antibodyAnti-MSTP039 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
